Coding

Part:BBa_K4853000:Design

Designed by: Bhavya Guduru   Group: iGEM23_Virginia   (2023-10-12)


Cyclase for nisin.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1265
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was codon optimized for E. coli BL21(DE3) using IDT Codon Optimization Tool.

Source

The L. lactis gene sequence was found through the NCBI nucleotide database1.

1 Lactococcus lactis strain N8 chromosome, complete genome - nucleotide - NCBI. National Center for Biotechnology Information. U.S. National Library of Medicine.

References